Liquid-phase electron microscopy is a technique that can be used to overcome one of the key limitations of electron microscopy – where the electron optics require a high vaccuum, and so the sample would need to be in a stable environment.
Researchers atÂ INM – Leibniz Institute for New Materials, SaarbrÃ¼cken, Germany, developed this technique in order to study proteins in their hydrated state. The team used a graphene liquid cell for the transmission of proteins during the microscopy, which resulted in an order of magnitude higher radiation tolerance of a sample of protein in comparison to amorphous ice.
Electron microscopy is one of the mainstream methods of analysing and investigating the structure of proteins. Traditionally, during this procedure the protein samples areÂ fixed, then stained with a metal to enhance their contrast, subsequently dried, embedded in plastic, cut in thin sections, and then imaged in the vacuum environment required for electron microscopy.Â
In more recent times, cryo-electron microscopy overcame the intense sample preparation method, by providing the means to study the protein in a native hydrated state – in amorphous ice. In order to resolve the structure, tens of thousands of ‘noisy’ images need to be taken (of identical areas) to resolve the structure of the protein.
The scientists used liquid-phase electron microscopy with unstained protein samplesÂ such as nanomaterials or cells in liquid, to image the structure of the protein. Until recently, it was debated whether this method would be better or worse than the method usingÂ amorphous ice.
In their recent publication, Dr Sercan Keskin and Dr Niels de Jonge from the INM-Leibniz Institute for New Materials demonstrated how the radiation tolerance wasÂ increased by an order of magnitude compared to a sample in ice. The microtubule sample was prepared in a graphene liquid cell, and the researchers mentioned that it was essential to use a low rate when the irradiation electron beam was applied.